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Cyclic nucleotides and calcium are crucial for axons to integrate extracellular signals required for axon pathfinding and to modulate axonal and synaptic competition. They are involved in a wide range of other signaling pathways. We aim to decrypt the codes used by axons and synapses to identify such cellular messenger signals as specific regulators of neuronal connectivity. FRET imaging enables following cellular messenger concentration in living cells. Other techniques including subcellular targeting of cAMP and calcium signaling blockers, and the use of light sensitive tools optogenetics make possible precise manipulations of these signals in time and space.

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